SOLICITATION NOTICE
66 -- Automated Fast Protein Liquid Chomatography System (FPLC) with High Pressure Liquid Chomatography (HPLC) Capacity
- Notice Date
- 6/12/2007
- Notice Type
- Solicitation Notice
- NAICS
- 334516
— Analytical Laboratory Instrument Manufacturing
- Contracting Office
- Department of Health and Human Services, National Institutes of Health, National Cancer Institute, Office of Acquisitions, 6120 Executive Blvd. EPS Suite 600, Rockville, MD, 20852, UNITED STATES
- ZIP Code
- 00000
- Solicitation Number
- Reference-Number-NCCI-70070-NV
- Response Due
- 6/19/2007
- Archive Date
- 7/4/2007
- Description
- The National Cancer Institute (NCI), Center for Cancer Research (CCR), Laboratory of Molecular Pharmacology (LMP), plans to enter into a sole source procurement for a AKTA Purifier 10 from GE Healthcare Bio-Sciences Corp., 800 Centennial Ave., Piscataway, NJ 08855. The North American Industry Classification System Code is 334516 and the business size standard is 500 employees. This acquisition will be processed under FAR Part 12 - Acquisition for Commercial Items and will be made pursuant to the authority in FAR 13.5 to use simplified procedures for commercial acquisitions. The GE Healthcare Bio-Sciences Corp. AKTA Purifier 10 is the only instrument known to the NCI researcher that has a Fast Protein Liquid Chromatography System (FPLC) system which will meet their research needs as detailed. However, if any interested party believes it can perform the requirement as detailed it may submit a statement of capabilities. The statement of capabilities and any other furnished information must be in writing and must contain material in sufficient detail to allow the NCI researcher to determine if the party can perform this requirement. An original and one copy of the capability statement must be received in the contracting office by 11:00 am EDT, on June 19, 2007. Faxed and emailed capability statements are NOT authorized. All questions must be in writing and may be emailed to Debbie Moore at dm170b@nih.gov or faxed (301) 402-4513. It is the vendor?s responsibility to call (301) 402-4509 and verify that questions were received. A determination by the Government not to compete this proposed contract based upon responses to this notice is solely within the discretion of the Government. Information received will be considered solely for the purpose of determining whether to conduct a competitive procurement. In order to receive an award from the NCI contractors must be registered in the Online Representations and Certifications Applications (ORCA). Please refer to http://orca.bpn.gov In addition; contractors must be registered in the Central Contractor Registration (CCR) www.ccr.gov. No collect calls will be accepted. JUSTIFICATION FOR OTHER THAN FULL AND OPEN COMPETITION Part I: Background information and Description of Acquisition Title: Purchase of an automated Fast Protein Liquid Chromatography System (FPLC) with High Pressure Liquid Chromatography (HPLC) capacity Purpose/Brief History: Two of the Laboratory of Molecular Pharmacology (LMP) groups will be concomitantly using this instrument for their research. The DNA replication group investigates how cells regulate the replication of their DNA. The group has identified DNA sequences likely to interact with cell cycle signaling molecules that control DNA replication. Subsequent studies have suggested that cells contain a protein that binds to this DNA sequence. The next step will be to isolate and characterize protein(s) involved in this binding activity. A protein purification system is essential for the isolation of such proteins or protein complex. The topoisomerase, DNA enzyme targeting group investigates the response of cells to anti-cancer drugs that perturb cell cycle progression and DNA replication. This group characterizes molecules that are subject to phosphorylation following treatment with anti-cancer drugs. This group will use the protein purification system to isolate and characterize the amino acid phosphorylation patterns of protein targets from cells treated with anti-cancer drugs. These studies are expected to yield new information regarding the regulation of cell growth under normal conditions and under conditions that can cause them to become cancerous and the response of cells to drugs used in the treatment of cancer. Understanding signals that lead from the cellular network that regulates growth to the genetic material may help clarify the differential sensitivity of particular cancer cells to specific drugs. Proteins that convey those regulatory signals might serve as targets for the development of novel, specific anti-cancer drugs. PART II: Facts and reasons to Justify Other than Full and Open Competition Statutory authority FAR 13.106(b)(1) b) Soliciting from a single source. (1) For purchases not exceeding the simplified acquisition threshold, contracting officers may solicit from one source if the contracting officer determines that the circumstances of the contract action deem only one source reasonably available (e.g., urgency, exclusive licensing agreements, or industrial mobilization). (2) For sole source acquisitions of commercial items in excess of the simplified acquisition threshold conducted pursuant to subpart 13.5, the requirements at 13.501(a) apply. Justification: In order for the LMP to further their mission they need to have the capabilities provided by the GE Healthcare Bio-Sciences AKTA Purifier 10. There is no other vendor capable of delivering an instrument with the needed capabilities of the AKTA Purifier 10 which are detailed below. The protein purification system must be based on fast flow liquid chromatography that includes the option for high pressure liquid chromatography which includes both fast flow and high pressure chromatograpy in a single purifier. To achieve both fast flow and high pressure chromatograpy we require a flow rate range of .001-10 ml/min at pressures ranging from 0-3700 psi. Because the system will be used concomitantly by two groups it must contain a way to select desired columns without removing the tubing and fittings by which the columns are plumbed into the flow path. The system must also include an automatic three-position injection valve (load, inject, wash positions), which will allow for automated sample loading and a UV detector able to simultaneously monitor 3 wavelengths that are settable from the controller. Laboratory space is limited, therefore the equipment can not be any larger than 500 mm widthX460 mm depth x 620 mm height. The system use must be able to perform separations based on a variety of sample characteristics, such as molecular weight, net surface charge, hydrophobicity, while retaining biological activity. Because of the heterogeneous sources we need a system capable of automating the running of large, open columns on the system, as well as a variety of gel filtration media. Because the resolution required may necessitate the use of very precise, shallow gradients, the system must have the following: 1. A two- pump gradient system, with dynamic mixing. 2. The entire wetted flow path of the system must be inert to halides. 3. A controller that allows time-based, volume-based, or Column-Volume based programming. The system must also allow for manual intervention while a programmed method is running. 4. At least two motorized valves, one for the column inlets, one for the column outlets. The desired column may be selected without removing the tubing and fittings by which the column is plumbed into the flow path, since such removal of fittings risks introduction of air into the column. 5. The system must include one automatic three-position injection valve (load, inject, wash positions), which will allow for automated sample loading. 6. A UV detector able to simultaneously monitor 3 wavelengths that are settable from the controller. The systems UV detection lamp must turn on and off automatically when the system is running saving on lamp life in addition to being automatically calibrated every time the lamp turns on. At each start-up each component must perform an automatic self-diagnostic test and calibrate appropriate settings ensuring top performance throughout the day-to-day operation. 7. The pumps, UV and conductivity monitors, valves, and fraction collector must all be specified for use in a cold room at 4 degrees C. 8. A system controller to control four motorized valves, fraction collector, dynamic gradient mixer, and two pumps must all be possible from the system controller. 9. The controller must allow the user to be entering details of the next purification at the same time the current separation is being done, or to simultaneously be integrating and evaluating the results of earlier runs. Data from the controlling system should be entered into word processing, spreadsheets, etc. that lie outside the operating program, without affecting currently running separations. 10. The controller must accept seven input signals (e.g. 3 UV wavelengths, conductivity, pH, temperature, pressure). It must be capable of plotting, and integrating these signals, as well as using them to trigger sub-routines in the programming when the signal exceeds a preset threshold, to allow the automation of multi-dimensional separations. 11. The system must have the ability to perform buffer blending and do this in a manner that is based on pKa and accounts for both ion concentration and temperature. 12. The system controller must allow for the automation of the optimization of separation parameters (e.g. sample volume, flow rate, gradient slope, buffer pH). 13. The system must be able to monitor column equilibration before and after sample injection and elution. 14. The documentation of each run should be automatic and exhaustive, so that for purposes of subsequent replication and eventual publication, no detail is left un-recorded. Post-run chromatographic analysis and reporting functions should also be included. 15. The system must have a settable pressure limit, which will stop the pump and generate an audible and visual warning in the event pressure exceeds the preset limit. 16. The controller must allow the user to set commands which will automatically reduce the flow rate if an over-pressurization occurs as sample is being applied or eluted from the column. Additional variables which should also be programmable to trigger sub-routines (e.g. new tube on fraction collector; valve changes; etc.) should include UV or conductivity signals. 17. The system must have the ability to be interfaced with an autosampler, which will be controlled from the system software. 18. The controller should permit automatic running of user-designated programs that change any single or several variables of choice, including: a. turning off the pump flow when a signal is received from the fraction collector indicating there no tubes remaining, so that valuable sample is preserved.; b. automatically and continuously record flow rate; auto-scaled UV signal ; actual and programmed conductivity of the eluent; eluent pH (with both ion concentration and temperature being accounted for); system pressure; accumulated time and volume of the run; all valve positions and changes in position; activity of a fraction collector c. the above recorded data should be available to be called up later for interpretation d. enabling post-run analysis of chromatograms, export of data to other spreadsheet programs, and generation of custom written reports. All data must be easily exportable into ASCII format for importation into alternative programs ie. Excel or Word for publication and presentation. e. The evaluation software must be capable of custom reports, HETP calculations, Sigma, Resolution and Histograms in addition to overlaying multiple runs and performing numerous manipulations. 19. The software must contain template protocols for purification of synthetic peptides, labeled synthetic oligonucleotides, native peptides, and protein fragments in addition to templates for general chromatographic techniques e.g. gel filtration, RPC, affinity, IEX , HIC and CIP. 20. Installation, training and online help must be provided by the vendor because it will be operated by a multiple users. The system must have at least a 1 year warranty on all parts and service.
- Record
- SN01316327-W 20070614/070612220416 (fbodaily.com)
- Source
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