SOURCES SOUGHT
B -- Salmon Reproduction, Imprinting, and Genetics Research
- Notice Date
- 5/8/2007
- Notice Type
- Sources Sought
- NAICS
- 541710
— Research and Development in the Physical, Engineering, and Life Sciences
- Contracting Office
- Department of Commerce, National Oceanic and Atmospheric Administration (NOAA), Western Region Acquisition Division, 7600 Sand Point Way, Northeast, Seattle, WA, 98115-6349, UNITED STATES
- ZIP Code
- 00000
- Solicitation Number
- Reference-Number-AB133F-06-CN-0133
- Response Due
- 5/18/2007
- Archive Date
- 6/2/2007
- Description
- NOAA intends to negotiate a sole source extension to Contract Number AB133F-06-CN-0133 with the University of Washington for the continuation of research on salmon reproduction, imprinting, and genetics research. Via the proposed extension, the contract period of performance would be extended until May 31, 2008. There are three tasks required for this requirement: 1) Assist with determining the effects of natural and artificial light on timing of spawning and development of secondary sex characteristics in sockeye salmon and effects of body growth on age 3 maturation and ovarian development in spring Chinook salmon. The following are required for this work: a) collection of tissues and plasma from experimental fish, b) measurement of plasma and pituitary gonadotropins, plasma sex steroids, and melatonin by radioimmunossay, c) measurement of pituitary ovarian gene transcripts (e.g. genes in the apoptosis cascade, vitellogenin receptor, zona radiata proteins, gonadotropin and growth hormone receptors, steroidogenic enzymes) from experimental fish by real time PCR, d) suppression subtraction hybridization cloning of ovarian genes, e) quantification of body color and morphology using digital cameras, color meter and computer image analysis, f) computer image analysis of gonad development from histological sections of gonads from experimental fish, g) ultrasound to identify maturing fish, and h) statistical analyses and interpretation of data collected from experimental fish. All analyses will be conducted at the Northwest Fisheries Science Center. Sockeye rearing will be done at the University of Washington, Big Beef Creek facility. 2) Assist with laboratory, behavioral and field assessments of olfactory imprinting by sockeye and Chinook salmon. To determine the critical period(s) for imprinting, juvenile salmon are being exposed to known odorants at key developmental stages and are being tested subsequently for development of long-term memories of these odorants. Results of these experiments will be important to determine the critical periods for imprinting for the offspring of captively-reared fish destined for release into natal rivers or lakes. The following are required for this work: a) maintain odor exposure equipment and odorants, b) conduct routine sampling of fish for tissue and physiological assays to assess smolting and imprinting, c) assist with behavioral assessments of imprinting in experimental stream channel, d) conduct electro-olfactogram (EOG) assessments of imprinted sockeye salmon and e) assist with molecular assessments of imprinting f) assist with carcass and redd surveys to assess imprinting and homing success of Chinook salmon. 3) Assist with assessing the degree of early male maturation of salmon in hatcheries in the Columbia River basin and determining effects of growth and photoperiod on the rate of early male maturation in Chinook salmon. This includes field trips to hatcheries and dams in Washington, Oregon and Idaho where fish are sampled for blood and gonads. In addition, controlled laboratory experiments are conducted in a research hatchery in Seattle where the effects of photoperiod, temperature and feeding rate on early male maturation are assessed. The following will be required for this work: a) conduct routine sampling of fish for tissue and blood to assess male maturation, b) organize and assemple of field sampling gear, c) collect and organize data on fish size and culture conditions, d) assist with experimental set-up and fish culture in the experimental hatchery, e) assist with analysis of plasma hormone levels including androgens and insulin-like growth factor I. Deliverables will be in the form of data collected from experiments and summarized in a FY08 annual report. The Government intends to negotiate a sole source extension to the existing contract with the University of Washington (UW), as it is believed that the UW is the only source with such unique expertise, qualifications, and experience to perform the work. All interested concerns must submit documentation in writing demonstrating the capability, expertise, qualifications, and experience to perform the necessary work to Contracting Officer Paul Reed at Paul.J.Reed@noaa.gov no later than May 18, 2007. It is requested that all submittals and inquiries be submitted via email to the stated address. See numbered note 22.
- Place of Performance
- Address: Seattle, WA
- Zip Code: 98195
- Country: UNITED STATES
- Zip Code: 98195
- Record
- SN01289472-W 20070510/070508220257 (fbodaily.com)
- Source
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