SOLICITATION NOTICE
99 -- MOUSE GPCR SIARRAY
- Notice Date
- 11/14/2006
- Notice Type
- Solicitation Notice
- NAICS
- 325414
— Biological Product (except Diagnostic) Manufacturing
- Contracting Office
- Department of Health and Human Services, National Institutes of Health, Nat'l Institute of Diabetes, Digestive, & Kidney Diseases, 2 Democracy Plaza, Suite 700W 6707 Democracy Blvd., MSC 5455, Bethesda, MD, 20892-5455, UNITED STATES
- ZIP Code
- 00000
- Solicitation Number
- NIH-NIDDK-07-059
- Response Due
- 12/8/2006
- Archive Date
- 12/23/2006
- Description
- The National Institutes of Health (NIH) National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK) has a requirement to purchase SMARTpool? siARRAY? libraries directed against (1) the GPCR genes (2) genes involved in apoptosis (3) Part III of the Druggable Genome (4) Standard siGENOME SMARTpool? reagent A collection from Dharmacon, 2650 Crescent Drive #100, Lafayette, CO 80026. SMARTpool? reagents are mixtures of four siRNA molecules (short, duplexed RNA oligonucleotides) designed to work in a highly defined manner to target a specific sequence within an mRNA such that cleavage of that mRNA is mediated, leading to a decrease in the protein encoded for by that mRNA. The sequence of each siRNA is unique, and extensive bioinformatic analysis has been carried out to ensure that each siRNA sequence will provide both maximum efficacy and target specificity. Dharmacon Inc. is the only source known to the NIDDK that has developed and continued to enhance their own proprietary algorithm that addresses these issues and thus the SMARTpool? siRNA libraries produced by them are unique in composition. NIDDK has worked with and optimized their system and assay using Dharmacon SMARTpool? siRNA reagents for over one year. Dharmacon has done significant research and publication on the design of functional siRNA and has developed a proprietary algorithm for the design of these reagents which has lead to a guarantee of 75% knockdown (the highest guarantee for any company in the industry) for the SMARTpool? siRNA reagent and 3 of the 4 individual siRNAs. Approximately 85% of SMARTpool? reagents silence target gene expression by 95% or more. Use of these reagents specifically from Dharmacon is necessary for the consistency and reproducibility in our experiments; these reagents, plus a large number of others that have been purchased in the past from Dharmacon, are part of an overall study in which direct comparison of the results is necessary. Changing variables at this time will be detrimental to current research in progress. One of the key elements to successful siRNA experiment is maximal and specific knockdown of the intended target gene. This becomes especially true in screening when a great number of targets are evaluated for functionality for further specific evaluation. The phenotype we are looking for can be hard to achieve and a high level of knockdown will be critical to the success of our experiments. In addition, Dharmacon uses a patented 2'ACE? chemistry for the synthesis of these reagents which minimizes the risk of degradation of the RNA. Specifications Required for All Sets: 1. 75% knockdown guaranteed for the SMARTpool? siRNA reagent and 3 of the 4 individual siRNAs (for follow up studies) measured by mRNA levels at 24 hours and normalized for transfection efficiency. 2. Reagents must be SMARTpool? siRNA reagents consisting of 4 duplexes designed against a specific target dried down in a well. 3. These Sets must be delivered within two weeks upon receipt of order. 4. Must use 2'ACE chemistry for the synthesis of RNA duplexes for the purpose of consistency and efficacy. 5. ON-TARGET? modification - selectively used to enhance selection of highly function siRNAs by inhibiting sense strand involvement in RISC-mediated knockdown. This selective use allows for silencing of highly conserved sequences while Specifications for siARRAY? siRNA libraries for GPCR, Protein Kinase, and Mouse Drug Targets Part III: 1. The SMARTpool? reagents must be delivered as 0.5 nmoles per well. 2. The GPCR library must consist of 474 SMARTpool? reagents 3. The Protein Kinase library must consist of 719 SMARTpool? reagents 4. Mouse Drug Targets Part III is a proprietary set of genes provided by Dharmacon Specifications for siGENOME reagent A collection: 1. The SMARTpool? reagents must be provided as 2 nmoles per well 2. This is a unique collection of targets developed in cooperation with Dharmacon. All responsible businesses that are capable of providing the Government with the items that are listed above may request a copy of the Request for Quote (RFQ) in writing or by FAX to 301-480-8501 to the Contracting Office prior to the closing date of this notice. It is anticipated that RFQ NIH-NIDDK-07-059 will be available fifteen (15) days after the publication date of this synopsis. Receipt of quotations will be due ten (10) days after the release of the RFQ. The anticipated award date is December 8, 2006. Request for copies of the RFQ must be submitted to the National Institute of Diabetes and Digestive and Kidney Diseases, 6707 Democracy Boulevard, Two Democracy Plaza, Room 774B, Bethesda, MD 20817 or via email: griffinv@mail.nih.gov to the Attention: V. Lynn Griffin. FAX quotations must contain offerors name, address, telephone number and fax number. FAX quotations will be accepted if dated and signed by an authorized company representative. All requests for quotations should have a valid email address.
- Place of Performance
- Address: BETHESDA, MD
- Zip Code: 20892
- Country: UNITED STATES
- Zip Code: 20892
- Record
- SN01180506-W 20061116/061114220212 (fbodaily.com)
- Source
-
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