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FBO DAILY ISSUE OF SEPTEMBER 09, 2004 FBO #1018
SOLICITATION NOTICE

B -- SAGE analysis

Notice Date

9/7/2004
 

Notice Type

Solicitation Notice
 

NAICS

541990 — All Other Professional, Scientific, and Technical Services
 

Contracting Office

Department of Health and Human Services, National Institutes of Health, National Cancer Institute, Research Contracts Br., 6120 Executive Blvd. EPS Suite 600, Rockville, MD, 20852
 

ZIP Code

20852
 

Solicitation Number

NCI-40162-NG
 

Response Due

9/21/2004
 

Archive Date

10/6/2004
 

Point of Contact

Malinda Holdcraft, Purchasing Agent, Phone (301) 402-4509, Fax (301) 402-4513, - Renita Smith, Contract Specialist, Phone 301-496-8612, Fax 301-480-0241,
 

E-Mail Address

holdcram@exchange.nih.gov, rs442i@nih.gov
 

Description

The National Institutes of Neurological Disorders and Stroke (NINDS), Human Embryonic Stem Cell Characterization Facility (HESCCF) plans to procure on a sole source basis services for Analysis of Gene Expression in Embryonic Stem Cells with Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Bunting-Blaustein Cancer Research Bldg, Room #262, 1650 Orleans St, Baltimore, MD 21231. The supplies and services herein are being procured in accordance with the simplified acquisition procedures authorized in FAR Part 13. The North American Industry Classification System Code is 541990 and the business size standard is $6M. The NIH Human Embryonic Stem Cell Characterization Facility (HESCCF) has been acquiring and growing human Embryonic Stem cells for almost one year. The HESCCF currently has many cultured lines and tested them for growth characteristics, viral contamination and karyotype integrity. The NIH effort will play a unique role in an international effort that involves studies by groups in other countries including Canada, Australia, Sweden and the United Kingdom. This collaboration will foster common standards of growth and characterization of undifferentiated human ES cells. The NIH HESCCF has an immediate role in two areas: 1) Comparison and characterization of stem cells on the NIH registry and; 2) Providing information and training on hES cells. This service relates to the first of these assigned functions. In this study, Serial Analysis of Gene Expression (SAGE) analysis will be used to compare the patterns of gene expression in four different subclones of two hES cell lines. Differences in patterns of gene expression will be used to characterize the stability of the parent cell lines. Sub-clones with distinct patterns of micro-array methods and gene expression will be analyzed for their growth and differentiation properties. The NIH Human Embryonic Stem Cell Characterization Facility is proposing to procure the Analysis of Gene Expression in Embryonic Stem Cells specifically from Dr. Robert Georgantas/Civin Lab, Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins. The SAGE technology was developed at Johns Hopkins Medical School and this group has experience with the recently developed long-SAGE method that makes them unusually well placed to obtain high resolution transcription maps from ES cells. The physical proximity of Johns Hopkins is another asset in this study as it will permit members of the NIH Human Embryonic Stem Cell Characterization Facility to interact directly with the Johns Hopkins team. This interaction may be particularly useful in developing maps of gene expression in small numbers of human ES cells with genetic or epigenetic changes. Therefore, the Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins is the only source known that can provide this service. This is not a request for competitive quotation. However, if any interested party believes it can perform the above requirement, it may submit a statement of capabilities. The statement of capabilities and any other furnished information must be in writing and must contain material in sufficient detail to allow NCI to determine if the party can perform this requirement. Capability statements must be received in the contracting office by 1:00 PM EST, September 21, 2004. If you have any questions they must be submitted in writing to Malinda Holdcraft, Purchasing Agent, at holdcram@exchange.nih.gov or by fax 301-402-4513. A determination by the Government not to compete this proposed requirement based upon responses to this notice is solely within the discretion of the Government. Information received will be considered solely for the purpose of determining whether to conduct a competitive procurement. Solicitation number must be cited on all correspondence, NCI-40162-NG.
 

Place of Performance

Address: Maryland

Zip Code: 20852

Country: USA
 

Record

SN00666134-W 20040909/040907211720 (fbodaily.com)
 

Source

FedBizOpps.gov Link to This Notice
(may not be valid after Archive Date)

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