SOLICITATION NOTICE
66 -- Liquid Chromatography System for Protein Purification
- Notice Date
- 8/25/2004
- Notice Type
- Solicitation Notice
- NAICS
- 334516
— Analytical Laboratory Instrument Manufacturing
- Contracting Office
- Department of Agriculture, Agricultural Research Service, Beltsville Area, ANRI, ASU, 10300 Baltimore Blvd., Bldg. 209 BARC EAST, Beltsville, MD, 20705
- ZIP Code
- 20705
- Solicitation Number
- 4011275125-00038
- Response Due
- 9/12/2004
- Archive Date
- 9/27/2004
- Point of Contact
- John Wilkinson, Purchasing Agent, Phone 301-504-5799, Fax 301-504-8696, - Nancy Woodhouse, Purchasing Agent, Phone 301-504-8012, Fax 301-504-8696,
- E-Mail Address
-
johnw@anri.barc.usda.gov, nancyw@anri.barc.usda.gov
- Description
- This is a combined synopsis/solicitation for commercial items identified below, prepared in accordance with the format in Far Subpart 12.6 as supplemented with additional information included in this notice. This announcement constitutes the only solicitation; offers are being requested and a written solicitation will not be issued. This solicitation number: 4011275125-00038, is issued as a Request for Quotation (RFQ), and incorporates provisions and clauses that are in effect through Federal Acquisition Circular 2001-24. This procurement shall be solicited on a unrestricted basis. The North American Industry Classification Code (NAICS) code is 334516. U.S. Dept. of Agriculture intends to procure a quantity of 1 each of the following item: ?Liquid Chromatography System for Protein Purification? with Product Number: 13443101?, (or equivalent), plus 8 other additional items as specified below - Manufactured by the vendor: ?Amersham Biosciences Corporation? - who are located at 800 Centennial Avenue, in Piscataway, New Jersey 08855-1327 - and they can be reached at phone # 1-800-527-3593. ?The following are U.S. Dept. Of Agriculture?s minimum requirements for the above item: Shall have the ability to purify biomolecules from complex starting materials derived from cell lysates, tissue homogenates, recombinant protein expression systems. Shall have the following sample characteristics: molecular weight, net surface charge, hydrophobicity, while retaining biological activity. Shall be able to automate the running of large, open columns on the system, and a variety of gel filtration media. Flow rate range shall be in the range from .05-20mls/per minute at pressures ranging from 0-700psi. Shall be a two-pump gradient system, with dynamic mixing. Shall have the entire flow path be inert to halides. The system?s pumps shall allow operation of the system at zero back pressure to ensure system compatibility with standard low pressure chromatography supports. The controller shall allow programs to be time-based, volume based, or column-volume based, and shall allow user intervention of a running method (e.g., change of flow rate) without stopping or pausing the run. The system shall include a fraction collector which is fully integrated into the system and fully controllable via the system control software. Shall have the ability to control nine motorized valves for column selection , buffer selection, flow reversal, and multi-dimensional chromatography. The motorized valves shall provide feedback to the control system to ensure proper orientation of all valves, and documentation of valve positions at all times. The valves shall be driven electronically and requires no external air pressure to operate. The system controller shall allow the end-user to be entering details of the next purification at the same time the current separation is being done, or to simultaneously be integrating and evaluating the results of earlier runs. The system controller shall allow up to nine motorized valves, chart recorder, fraction collector, dynamic gradient mixer, air sensors, and three pumps. The pumps, UV and conductivity monitors, valves and fraction collector shall all be specified for use in a cold room at 4 degrees Celsious. The controller shall accept seven input signals (e.g. UV Wavelength, conductivity, pH, temperature, pressure.) The system controller shall be capable of plotting, and integrating these signals, as well as using them to trigger sub-routines in the programming when the signal exceeds a preset threshold, which shall allow the automation of multi-dimensional separations. The system shall include one automatic three-position injection valve (load, inject, and wash positions), which shall allow for sample loading onto the column. The system controller shall allow for automation of the optimization of separation parameters (e.g. sample volume, flow rate, and gradient slope- which shall allow a small amount of sample to be consumed in optimizing the purification scheme, after which the best regime can be scaled up on the same LC system. Column equilibration before and after sample injection and elution shall be capable of being monitored , with a feed-back loop to the control system, so the end-user can be certain the equilibration is completed, without consuming excess volume of sometimes expensive buffers. Documentation of each run should be automatic and exhaustive, so that for purposes of subsequent replication and eventual publication, no detail is left un-recorded. Post-run chromatographic analysis and reporting functions shall also be included. In order to protect our columns, the system shall have a definable pressure limit system which shall stop the pump and generate and audible and visual warning, in the event pressure exceeds the preset limit. The controller shall also allow the end-user the option of including in their chromatographic methods program, which commands shall automatically reduce the flow rate if an over-pressurization occurs as sample is being applied or eluted from the column. Additional variables which should also be programmable to trigger sub-routines (e.g. new tube on fraction collector; valve changes) which shall include UV or conductivity signals. The controller shall permit automatic running of up to 99 different chromatographic programs in a user-designated sequence; it shall turn off the pump flow when a signal is received from the fraction collector indicating there are no tubes remaining, so that valuable sample is preserved; the system shall automatically and continuosly be able to record throughout each chromatographic run with the following parameters: flow rate; auto-scaled UV signal; actual AND programmed conductivity of the eluent; eluent PH; system pressure; accumulated time of run; all valve positions and changes in position; activity of a fraction collector, and this shall allow these recorded types of data to be called up later for interpretation, and to aid in future replication of results; it shall allow for post-run analysis of chromatograms, export of data to other spreadsheet programs, and generation of written reports. Shall include installation and training for the above system. The following 8 lined items are Accessories that go with the above system: (1) Product Number: 18103211, Rinsing Tube, Quantity of 1 each; (2) Product Number: 18110841, PV-908, 25MPA, Quantity of 2 each; (3) Product Number: 18608312, Rack B, 12 MM, FRAC-950 Tubes, Quantity of 1 each; (4) Product Number: 18672700, Pump P-960, Quantity of 1 each; (5) Product Number: 18117584, Air Sensor A-912N Kit, Quantity of 1 each; (6) Product Number: 18111381, Superloop, 10 ML, Quantity of 1 each; (7) Product Number: 18102385, Superloop, 150 ML, Quantity of 1 each; and (8) Product Number: 18115424, Akta User Kit, Quantity of 1 each. ?F.O..B. Destination? with inside delivery to the U.S. Department of Agriculture in Beltsville, Maryland 20705. Delivery is required 60 days after award. This solicitation incorporates the following FAR clauses, provisions, and addendums: 52.212-1 Instructions to Offerors-Commercial Items; 52.212-2 Evaluation-Commercial Items; 52.212-3 Offeror Representations and Certifications-Commercial Items; 52.212-4 Contract Terms and Conditions-Commercial Items; 52.212-5 Contract terms and Conditions Required to Implement Statutes or Executive Orders-Commercial Items. In Paragraph (B) of 52.212-5, the following apply: 52.203-6, 52.222-21, 52.222-26, 52.222-35, 52.222-36, 52.222-37, 52.225-3, 52.225-13, 52.232-34, and 52.222-70. COMPLIANCE WITH VETERANS EMPLOYMENT REPORTING REQUIREMENTS (JAN 1999)(DEVIATION)(USDA). (a) The offeror represents that, if it is subject to the reporting requirements of 38 U.S.C. 4212 (d) (i.e., the VETS-100 report required by FAR Clause 52.222-37, Employment Reports on Disabled Veterans and Veterans of the Vietnam Era), it has[ ], has not[ ], submitted the most recent report required by 38 U.S.C. 4212 (d). (b) An Offeror who checks ?has not? may not be awarded a contract until the required report is filed. CONTRACT PAYMENT (MANDATORY INFORMATION FOR ELECTRONIC FUNDS TRANSFER) Electronic Funds Transfer compliance with 31 CFR 208. To Facilitate EFT for contract payments, FAR Clauses 52.232-34 and 52-232-35 (31 CFR Part 208) requires the submission of information by the contractor to the payment office. Upon Award, the contractor shall contact the Miscellaneous Payments Section at the National Finance Center (NFC) to initiate the enrollment process of the Vendor Express Payment (VXP), which will result in a number being assigned to the contractor for payment under EFT. NFC can be reached at phone number 1-800-421-0323 or at WWW.NFC.USDA.GOV. All offerors must include copies of 52.212-3 (Representations and Certifications) and 52.222-70(Compliance with Veterans Employment Reporting Requirements). The Magnitude/Range of the above requirement is greater than $25,000 and under $100,000. Faxed Quotations are acceptable and shall be faxed to John Wilkinson to fax number (301) 504-8696 on or before September12, 2004. All responses will be evaluated in order to determine the ability to meet the above stated capabilities. Responses shall include technical specifications, literature, brochures and other such information corresponding to each minimum required item, which demonstrates the capabilities of the offeror?s equipment. Offerors shall identify where the offered item meets or does not meets each of the Government?s functional and performance minimum specifications listed herein. The Government will award a contract resulting from this solicitation to the responsive/responsible offeror whose offer conforming to the solicitation will be most advantageous to the Government. The anticipated award date is September 14, 2004. All responsible sources may submit a quotation, which shall be considered by the Agency.
- Place of Performance
- Address: USDA,ARS,BA,PSI,MPPL;, 10300 BALTIMORE AVENUE;, BUILDING 004, RM. 227, BARC-WEST;, BELTSVILLE, MARYLAND,
- Zip Code: 20705
- Country: USA
- Zip Code: 20705
- Record
- SN00653481-W 20040827/040826074434 (fbodaily.com)
- Source
-
FedBizOpps.gov Link to This Notice
(may not be valid after Archive Date)
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