Loren Data's SAM Daily™

FBO DAILY ISSUE OF AUGUST 29, 2003 FBO #0638
SOLICITATION NOTICE

99 -- Sequencing Services for 20,000 ESTs from a normalized cDNA library synthesized from Boophilus microplus

Notice Date

8/27/2003
 

Notice Type

Solicitation Notice
 

Contracting Office

Department of Agriculture, Agricultural Research Service, Southern Plains Area Office, 7607 Eastmark Drive, Suite 230, College Station, TX, 77840
 

ZIP Code

77840
 

Solicitation Number

R69-7MN1-03
 

Response Due

9/23/2003
 

Point of Contact

Michael Douglas, Contract Specialist, Phone (979) 260-9480, Fax (979) 260-9413, - Michael Douglas, Contract Specialist, Phone (979) 260-9480, Fax (979) 260-9413,
 

E-Mail Address

mdouglas@spa.ars.usda.gov, mdouglas@spa.ars.usda.gov
 

Description

This is a combined synopsis/solicitation for commercial items prepared in accordance with the format in FAR subpart 12.6, as supplemented with additional information included in this notice. This announcement constitutes the only solicitation; proposals are being requested and a written solicitation will not be issued. The solicitation number is R69-7MN1-02 and is issued as a request for quotation (RFQ). This solicitation is a small business set-aside; The NAICS is 334516, business size 500 employees. This solicitation document incorporates those provisions and clauses in effect through Federal Acquisition Circular 01-15. The USDA, Agricultural Research Service, requests the procurement of sequencing services for 20,000 ESTs from a normalized cDNA library synthesized from Boophilus microplus. The use of modern molecular approaches for designing novel methods of tick control require increased knowledge in the identification and quantification of expressed genes in the tick and the role specific genes may play in tick development and survival of various stresses encountered by the tick, including dessication, cold, and pesticide exposure. As an initial step in assembling a database of expressed tick genes, a tick cDNA library has been synthesized which contains genes activated by various environmental perturbations and genes expressed in various lifestages of the tick. The objective of this research is to obtain sequence information from genes expressed in the tick with the longer-term goal of relating the expression or activation of specific genes to specific environmental cues. Approach: Expressed sequence tag (EST) data will be compiled into a database of expressed tick genes. Clones from the tick cDNA library will be sequenced from both the 5' and 3' ends using standard sequencing primers. The sequence information will be auto-annotated and putative gene identification assigned. The EST database will be used to build a gene index for Boophilus microplus, assembling sequences into tentative consensus sequences and singletons. Specifications: (1) receive and store the normalized cDNA library; (2) plate the library on selective media, picking bacterial colonies containing cDNA inserts, and preparing DNA for sequencing; (3) Perform 5' and 3' sequencing (20,000 reactions in total); (4) Auto-annotate sequence data, searching for putative open reading frames; (5) Provide sequence information in flat files with putative gene identification when possible; (6) Build a gene index of Boophilus microplus using these ESTs. The Sequencing information shall be delivered to the USDA, Agricultural Research Service, 2700 Fredericksburg Rd, Kerrville, TX 78028. The Government ?REQUIRES? delivery no later than 90 days after receipt of order, however, each offeror shall include their proposed delivery schedule as part of their quotation. Acceptance and FOB point are destination. In order to be considered for award offerors shall provide a quotation on company letterhead detailing the unit pricing; descriptive literature, brochures, and five references for the proposed item. References must have received the proposed item within the last three calendar years (Government references preferred but will accept commercial references) shall also be provided for the product quoted. 52.252-2 CLAUSES INCORPORATED BY REFERENCE; FAR 52.212-4 CONTRACT TERMS AND CONDITIONS - COMMERCIAL ITEMS; FAR 52.212-5 CONTRACT TERMS AND CONDITIONS REQUIRED TO IMPLEMENT STATUTES OR EXECUTIVE ORDERS - COMMERCIAL ITEMS applies, with the following clauses from (a) 52.222-3, Convict Labor; 52.233, Protest after award, and (b) FAR 52.219-8, Utilization of Small Business Concerns; FAR 52.222-26, Equal Opportunity; FAR 52.222-35, Equal Opportunity for Special Disabled Veterans, Veterans of the Vietnam Era, and Other Eligible Veterans; FAR 52.222-36, Affirmative Action for Workers with Disabilities; FAR 52.222-37, Employment Reports on Special Disabled Veterans, Veterans of the Vietnam Era, and Other Eligible Veterans; FAR 52.222-19, Child Labor - Cooperation with Authorities and Remedies; FAR 52.225-13, Restrictions on Certain Foreign Purchases; FAR 52.232-34, Payment by Electronic Funds Transfer - Other than Central Contractor Registration; applicable to this acquisition: FAR 52.252-1 SOLICITATION PROVISIONS INCORPORATED BY REFERENCE; FAR 52.212-1 INSTRUCTIONS TO OFFERORS - COMMERCIAL ITEMS, FAR 52.212-2 EVALUATION - COMMERCIAL ITEMS, the significant evaluation factors, in the relative order of importance, are (i) technical capability of the item offered to meet the Government requirement; (ii) past performance; and (iii) price (based on F.O.B. Destination), where technical and past performance are more important than price. Offerors shall include a completed copy of the provision at FAR 52.212-3 OFFEROR REPRESENTATIONS AND CERTIFICATIONS - COMMERCIAL ITEMS, with this offer; a copy of this provision may be obtained at http://www.arnet.gov/far. Any amendment and documents related to this procurement will be available electronically at the Internet site: http://www.eps.gov. Potential offerors will be responsible for downloading their own copy of these documents (if any). It is the offerors responsibility to monitor the site for release of the solicitation and any related documents. Furnish quotations to Michael Douglas, Contracting Officer, USDA, ARS, SPA, 7607 Eastmark Drive, Suite 230, College Station, Texas, 77840, no later than 3:00p.m., September 23, 2003. Quotations my be provided by facsimile to (979) 260-9413 if desired. Additional information may be obtained by contacting the contracting officer at (979) 260-9480 (voice) or email below.
 

Place of Performance

Address: 2700 Fredericksburg Road, Kerville, Texas

Zip Code: 78028

Country: USA
 

Record

SN00413334-W 20030829/030827213217 (fbodaily.com)
 

Source

FedBizOpps.gov Link to This Notice
(may not be valid after Archive Date)

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