SOLICITATION NOTICE
A -- Non-personal research services for Cohclear Biochemical response to traumatic noise exposure for 1 year plus 1 option year for the period 01 Apr 2002 to 31 Mar 2003.
- Notice Date
- 3/22/2002
- Notice Type
- Solicitation Notice
- Contracting Office
- Department of the Navy, Bureau of Medicine and Surgery, NMC San Diego, Bldg 1 38400 Bob Wilson Drive, San Diego, CA, 92134-5000
- ZIP Code
- 92134-5000
- Solicitation Number
- N00259-02-T-0031
- Response Due
- 3/26/2002
- Archive Date
- 4/10/2002
- Point of Contact
- loida toledo, lead purchasing agent, Phone 619-532-8122, Fax 619-532-5596, - Joan Balazs, SPVY, Contract Specialist, Phone 619-532-5944, Fax 619-532-5596,
- E-Mail Address
-
lctoledo@nmcsd.med.navy.mil, jbalazs@nmcsd.med.navy.mil
- Description
- This notice is provided on a sole source basis, with an intent to award a firm fixed price order with Baylor University Medical Center, Insititute of metabolic Disease for a period of one year (base year) plus one option year. This is a combined synopsis/solicitation for commercial items, prepared in accordance with the format in FAR subpart 12.6 as supplemented with additional information included in this notice. This announcement constitutes the only solicitation, quotations are being requested and a written solicitation will not be issued. The solicitation number, N00259-02 -T-0031 is issued as a request for quotations(RFQ). Clin 0001: Non-personal service contract for Research services for the period 01 Apr 2002 to 31 Mar 2003. Qty: 1; Unit of issue: year . Clin 0002: Option year 1 for the period 01 Apr 2003 to 31 Mar 2004. The duties to be performed shall be in accordance with the statement of work.The statement of work reads. INTRODUCTION: The Department of Defense Spatial Orientation Center of Naval Medical Center, San Diego staff conducts ongoing projects in auditory and vestibular research. ABREVIATIONS: DOD = Department of Defense, ONR = Office of Naval Research, NMCSD = Naval Medical Center San Diego, BU = Baylor University, GPOC = Government Point of Contact, DSOC = DOD Spatial Orientation Center, ROM = reactive oxygen molecules, FR = free radicals, SPL = sound pressure level, mL = micro liter, kHz = kilohertz, HPLC = High Pressure Liquid Chromatography, COL = Colonel, MC = Medical Corp, FY = Fiscal Year. SCOPE: Contractor will conduct research into cochlear biochemical responses to intense noise exposures in support of funded investigations under the ONR Military Operational Medicine program. This program encompasses research into developing countermeasures for the prevention of tissue/organ damage prior to exposures to high-energy sources (loud sounds) and to develop methods for rescue and treatment after exposure to exotic energy sources. This work entails performing a series of experiments over three years to: (1) understand the cochlear intracellular biochemical response to damaging sound energy, (2) to identify and delineate the reactions involved with glutathione metabolism and reactive oxygen molecules (ROM) and free radicals (FR) produced by oxidative stress in response to acoustic over-exposure, and (3) utilize our recent antioxidant technology to increase hair cell glutathione or antioxidant defenses to reduce the formation of and damage caused by FRs and ROM molecules in order to prevent hair cell damage or death caused by traumatic noise. The first year encompasses three experiments (A, B and C). Experiment A involves studying groups of chinchillas under no noise conditions to characterize baseline biochemical and metabolic responses of the auditory hair cells. Experiment B entails exposing the same number of animals, first to one hour of no noise for baseline measurements and then for four hours of continuous noise at 105 dB SPL octave band noise centered at 4 kHz. In Experiment C animals will again undergo one hour of baseline measures (no noise) then be noise-exposed for four hours but prior to noise exposure a glutamate antagonist will be administered to study its capability to reduce perilymphatic glutamate levels and counteract excitotoxicity. The treatment animals will be compared to saline injected noise-exposed animals, which will serve as controls. In each experiment cochlear perilymph fluid will be sampled using micro dialysis technology. Perilymphatic samples are taken at a rate of 1mL per minute giving three 20 mL specimens per hour. As control reference for perilymphatic metabolism and to also monitor the stress level of the whole animal, a sample of brain and blood plasma will be taken and measured for the same metabolites. Year two will investigate the efficacy of different glutathione prodrugs that can enhance hair cell glutathione levels in protecting against noise-induced increases in ROM molecules and FRs. Again cochlear perilymph, brain tissue and blood plasma samples will be taken at the same time schedule. Year three will be very similar to year two. However, this set of experiments will investigate the effects on glutathione and its metabolites as well as the ROM molecules and FRs measured previously, but in these sets of experiments animals will be pre-treated with agents that will enhance the survival of hair cell mitochondria. Again blood plasma, brain tissue and perilymphatic samples will be taken in the time schedule described above. TECHNICAL REQUIREMENTS: In all 2 years and for all experiments, the contractor will receive shipments of frozen blood plasma, brain tissue and perilymphatic samples as collected by micro dialysis sampling. After careful calibration of equipment, quantification of the following metabolites: glutamate, cysteine, reduced and oxidized forms of glutathione, malendialdehyde and dihydroxybenzoate will be measured from each perilymphatic aliquot and from each brain and plasma specimen. The contractor will assess the biochemical events taking place in the cochlea during quiet conditions and then following damaging sound exposure utilizing high pressure liquid chromatography (HPLC). This study will elucidate the cellular biochemical response of hair cells to noise-induced damage and investigate the capability of glutathione enhancing and mitochrondrial protectants to prevent noise-induced cell death (apoptosis). The brain tissue and blood plasma samples will be used for controls and to determine the effect of the noise stress on the state of the animal. Duties: The contractor shall perform a full range of research procedures, within the scope of his training and experience, on site using Baylor University Medical Center furnished facilities, supplies and equipment. Workload occurs as a result of scheduled and unscheduled requirements for research activities. Primary workload is a result of experiments generated to pursue the specific aims of research protocols funded by the Office of Naval Research and the DOD Spatial Orientation Center (DSOC), Naval Medical Center, San Diego (NMCSD). Contractor is responsible for a full range of experimental requirements in neurochemical research including the planning and execution of research within the personnel and equipment capabilities of the Baylor University facility, and the quality and timeliness of experimental records and reports required to document procedures performed and services provided. The research worker shall monitor supply and material levels and work with appropriate personnel to reorder supplies, and materials as needed to support ongoing and planned research. Contractor is responsible for screening for and identifying with chemical processing and high pressure liquid chromatography (HPLC) the following metabolic compounds from perilymphatic dialysates, brain and blood plasma samples: oxidized and reduced glutathione, glutamate, cysteine, dihydroxybenzoate and malendialdehyde. In year one it is projected that there may be between 2700 and 2800 samples of perilymph, 90 tissue samples of brain tissue and 90 specimens of blood plasma to analyze. Year two contractors may analyze between 3600 and 3700 perilymph samples, 120 brain tissue samples and 120 blood plasma specimens. For year three it is projected that there may be between 2700 and 2800samples of perilymph, 90 tissue samples of brain tissue and 90 specimens of blood plasma to analyze. Contractor will provide oversight and guidance for all procedures and data analysis of all experiments at the Baylor University Medical Center, Institute of Metabolic Disease site. Contractor will also work closely with COL. Richard Kopke, MC, USA, NMCSD Program Manager who has scientific and technical responsibility for this research project, to coordinate investigational efforts, trouble shoot problems that develop and to collate data from the different work sites. Contractor will meet with COL Kopke twice in year one at the NMCSD site. First meeting will be prior to the start of the project to assist NMCSD staff in the setup, calibration and training in the operation of the micro dialysis equipment and to finalize plans and coordinate timing of collection and shipping of biological specimens. A second meeting is planned near the end of the first year to determine the outcomes and trends of data, analysis of findings and planning for the second year?s research. Meetings after year one will be coordinated between contractor and COL Kopke. Contractor will organize all data collected for this project in a database/spreadsheet format that is compatible with the NMCSD data manager. Contractor will organize data for publications and oversee the preparation of manuscripts and summary figures resulting from the work performed in this grant proposal. Contractor will perform all of the HPLC preparation and analysis of inner ear perilymphatic specimens. Funding: NMCSD through ONR funding agrees to pay Baylor University for 2 years . Personnel costs are allocated for a research associate?s salary. Supplies are for High Pressure Liquid Chromatography items, reagents and chemical supplies. Fringe costs are used to cover health insurance costs. Indirect costs are fees charged by Baylor University. The total payment for personnel, services, fringe, supplies and institutional indirect costs shall be paid out for 2 years. Contractor shall maintain current accounts, records, and other evidence supporting all its expenditures chargeable to NMCSD under this contract for at least 12 months after completion or termination of this contract. Contractor shall provide NMCSD with a report within four months of completing performance under this contract. Reports and Publications: Contractor shall submit quarterly reports to NMCSD during the term of this contract on the progress of its research and the results obtained, to the extent reasonably requested. Contractor shall submit a final report of its results, including a listing of all Subject Inventions, to NMCSD within four months after completing the work under this contract. Contractor and NMCSD agree to confer and consult prior to the publication of research data. Prior to submitting a manuscript(s) for review (or without review) which contains the results of research under this contract, each site must have ample opportunity and time to review such proposed publication and to file patent applications a timely manner. COL Richard Kopke, MC, USA, NMCSD Program Manager contractor are the responsible individuals for review of materials and the designated point of contacts for each respective site. QUALIFICATIONS: Contractor is an expert in the field of biology, neuropharmacology and neurochemistry of sensory tissues and methodological assessment of the nervous system especially in response to damage, trauma or disease states. Contractor has completed a doctoral degree in the Neurochemistry. Has over 20 years experience in the area of research interest. Contractor must have medical research laboratory with lab space, equipment, technical and administrative staff to support this project. Expertise in the neurochemistry and neuropharmacology of the brain and central nervous system. Has a proven publication tract in the area of biology and neurochemistry of neurosensory tissue. Contractor is an internationally recognized researcher in the area of neurobiology and neurochemistry. SECURITY: o security clearance is needed for contractor. PLACE OF PERFORMANCE: Contractor will work 100% of the time at the contractor?s site. INSPECTION AND ACCEPTANCE: Receives supervision from the Government Point of Contact (GPOC). Work is reviewed by GPOC for adequacy of method, completeness, scientific importance, and for applicability to ONR and DOD Spatial Orientation Center?s (DSOC?s) research objectives. Government Point of Contact will be: COL Richard Kopke, MC, USA, NMCSD Program Manager at (619) 532-9604 or pager (877) 774-9106 or FAX (619) 532-6088 . The solicitation document and incorporated provisions and clauses are those in effect through FAC 2001-05. The following clauses and provisions are applicable and will be incorporated into the solicitation by reference, and will be available in full text upon request. This acquisition incorporates the following FAR clauses: 52.212-1 INSTRUCTIONS TO OFFERORS-COMMERCIAL ITEMS(Oct95);52.212-3 OFFEROR REPRESENTATIONS AND CERTIFICATIONS-COMMERCIAL ITEMS (Oct 95) must be completed and submitted along with the proposal (please contact Loida Toledo at (619)532-8122 if a copy is needed; in accordance with FAR 52.212-5 CONTRACT TERMS AND CONDITIONS REQUIRED TO IMPLEMENT STATUTES OR EXECUTIVE ORDERS-COMMERCIAL ITEMS(Aug 96), the following clauses are incorporated by reference in paragraph (b): 52.222-26 EQUAL OPPORTUNITY ( E.O. 11246); 52.222-35 AFFIRMATIVE ACTION FOR SPECIAL DISABLED AND VIETNAM ERA VETERAN (38 U.S.C. 4212); 52.222-37 EMPLOYMENT REPORTS ON SPECIAL DISABLED VETERANS AND VETERANS OF THE VIETNAM ERA(38 U.S.C. 4212); 52.222-36 AFFIRMATIVE ACTION FOR HANDICAPPED WORKERS(29 U.S.C. 793); 52.212-4 CONTRACT TERMS AND CONDITIONS COMMERCIAL ITEMS(OCT 95). DFARS 252.204-7004, REQUIRED CENTRAL CONTRACTOR REGISTRATION (MAR 1989); FAR 52.232-36, PAYMENT BY THIRD PARTY (MAY 1999). FAR 52.232-33 , MANDATORY INFORMATION FOR ELECTRONIC FUNDS TRANSFER PAYMENT (AUG 1996). FAR 52.217-5 EVALUATIONS OF OPTIONS(JUL 1990); 52.217-8, OPTION TO EXTEND SERVICES (AUG 1989); 52.217-9 OPTION TO EXTEND THE TERM OF THE CONTRACT (MAR 1989) FAR 52.212-2 , EVALUATION ?COMMERCIAL ITEMS ( 1/99). The following factors shall be used to evaluate offers: 1) Past performance 2) personnel qualifications based on SOW 3) Price. Past performance and personnel qualifications based on SOW when combined have more importance that cost or price. Prior to Contract Award, the government reserves the right to review the resumes of contractor employees performing under the contract solely for the purpose of ascertaining their qualifications relative to the personnel qualifications terms of the contract. Accordingly, the contractor shall furnish such resumes and all professional requirements as stated in the Statement of Work ( SOW) to the Purchasing Agent along with their quotes. All responsible sources may submit a quote which shall be considered. See note 22.
- Place of Performance
- Address: Baylor University Medical Center, Institute of Metabolic Disease, 3812 Elm Street, Dallas, Texas
- Zip Code: 75226
- Zip Code: 75226
- Record
- SN00047298-W 20020324/020322213410 (fbodaily.com)
- Source
-
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